Thesis - Open Access
Master of Science (MS)
The pH 4. 4 supernatant from raw (unheated) soybean meal was separated into 5 fractions on a Sephadex G-25 column in efforts to isolate the animal growth inhibitors present soybeans. Each fraction was added to the diet of growing mice and growth inhibition (GI) activity was determined by comparing their_ growth rates to g1"'owth rates achieved on an autoclaved soybean meal diet. Fractions I and II each contained about 50% of the GI activity, with a trace in fraction III and none in fractions IV and V. Only fraction I contained trypsin inhibitor and caused pancreatic enlargement. Fraction I was further separated into 2 fractions (Ia and Ib) on a Sephadex G-50 column. Fraction Ib contained trypsin inhibitor and caused pancreatic enlargement. Both fractions Ia and Ib contained GI activity, with most of the GI activity present in Ib. Fraction II was further separated into 2 fractions (IIa and IIb) on a phosphocellulose column eluted with a KCl gradient. Most of the GI activity was present in fraction IIb. Amino acid analysis of G-25 - II indicated all amino acids present, with the sulfur-containing amino acids, methionine and half cystine, present in only very small amount.
Library of Congress Subject Headings
Soybean as feed
Plant growth inhibiting substances
Includes bibliographical references (pages 31-36)
Number of Pages
South Dakota State University
Uckert, Joseph Richard, "Partial Characterization of Animal Growth Inhibitors from Soybeans" (1973). Theses and Dissertations. 1279.