•  
  •  
 

Faculty Mentor

Fedora Sutton

Abstract

Search of international DNA database, Genbank, produced eight barley cDNA EFlα sequences with unique accession numbers. The length of these sequences varied from an incomplete 502 bp to a full length 1646 bp encoding a 447 amino acid protein. The knowledge that there are four EF-lα gene family members in barley led us to query whether all four genes were represented in the group of eight database submitted sequences. Also of great importance was the correct assignment of gene family member to its mRNA and protein expression profiles. Before we could begin to address the expression studies, we undertook the task of ascribing to the published cDNA sequences its grouping in the gene family. Bioinformatic tools were used to identify the 5' and 3' UTRs of the cDNA sequences. The 5' & 3' UTR comparisons revealed that sequences with accession numbers LI 1740 & Z23130 were identical. Thus these were designated as derived from the EF-lαA gene. Sequences BQ294517, BQ739965, & Z50789 represented the same gene family member designated EF-lα B. BQ740018 was derived from a third gene designated EF-lα C. BQ740062 also differed enough to be classified as derived from the fourth member of the gene family herein designated EF-lα D. Absence of 5' and 3' UTRs, for BQ739951 prevented designation with respect to gene family. Thus it is placed in the family group U (unknown).

Share

COinS
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.