Document Type

Article

Publication Version

Version of Record

Publication Date

12-2016

Keywords

Caryophyllaceae, microsatellites, plant–animal interaction, polyploidy, Silene stellata

Abstract

Premise of the study:We designed and tested microsatellite markers for the North American native species Silene stellata (Caryophyllaceae) to investigate its population genetic structure and identify selection on floral design through male reproductive success.

Methods and Results: A total of 153 candidate microsatellite loci were isolated based on next-generation sequencing. We identified 18 polymorphic microsatellite loci in three populations of S. stellata, with di- or trinucleotide repeats. Genotyping results showed the number of alleles per locus ranged from six to 45 and expected heterozygosity ranged from 0.511 to 0.951. Five of these loci were successfully amplified in S. virginica and S. caroliniana and were also polymorphic.

Conclusions: The microsatellite markers reported here provide a valuable tool for paternity analysis in S. stellata. They will also be useful for investigating the population genetic structures of S. stellata and related species.

Publication Title

Applications in Plant Science

Volume

4

Issue

12

Pages

5

Format

application/pdf

Language

en

DOI of Published Version

10.3732/apps.1600117

Publisher

Botanical Society of America

Rights

This article is a U.S. Government work and is in the public domain in the USA.

Creative Commons License

Creative Commons Attribution-Share Alike 3.0 License
This work is licensed under a Creative Commons Attribution-Share Alike 3.0 License.

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