Effects of yeast culture supplementation on systemic and polymorphonuclear leukocyte mRNA biomarkers of inflammation and liver function in peripartal dairy cows

Document Type


Publication Date



American Dairy Science Association


Journal of Dairy Science




Suppl. 1






transition period, inflammation, yeast culture


Feeding yeast culture additives has been attributed to health benefits during the transition period of dairy cows. The objective of this study was to evaluate the effects of feeding a commercial yeast culture (Cellerate Culture Classic HD (YC); Phibro Animal Health, Teaneck, NJ) on blood biomarkers of inflammation and liver function, and gene expression in polymorphonuclear leukocytes (PMNL) in dairy cows during the transition period until 50 DIM. Forty Holstein dairy cows were used in a randomized complete block design from −30 to 50 DIM and blocked according to parity, previous lactation milk yield, and genetic merit. At −30 DIM cows were assigned to a basal diet plus a top-dressed of either 114 g/d of ground corn (CON; n = 20) or 100 g/d of ground corn and 14 g/d YC (n = 20). Cows received the same close-up diet from −30 to calving (0.63 Mcal/kg DM and 12.3% CP) and lactation diet (0.73 Mcal/ kg DM and 15.6% CP). Blood samples were collected at −30, −15, 7, 14, and 30 DIM to evaluate biomarkers related to liver function and inflammation. Blood samples for PMNL gene expression were collected at −30, −15, 5, 10, and 30 DIM. The mRNA in PMNL will be analyzed for genes associated with inflammation, cellular receptor, and oxidative stress. Data were analyzed using the MIXED procedure of SAS. Neither prepartal nor postpartal DMI was affected (P ≥ 0.40) by dietary effects, whereas ECM tended (P = 0.07) to be greater in YC than CON cows. Postpartal diseases and conditions were similar (P = 0.31) between treatments. There was a trend (P = 0.07) for a Diet × Time interaction in GGT (γ-glutamyl transferase), where greater (P < 0.01) GGT was observed in YC than CON cows at 30 DIM. Negative acute-phase proteins (APP), albumin (P = 0.06) and paraoxonase (P = 0.12) tended to be lower in YC than CON cows. The positive APP, haptoglobin[CN1], tended (P = 0.12) to be higher in YC than CON. Results do not demonstrate a conclusive dietary effect on liver function or inflammation, while a modest improvement in milk production was observed in YC cows.