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Document Type

Thesis - University Access Only

Award Date


Degree Name

Master of Science (MS)

Department / School

Plant Science

First Advisor

Jose L. Gonzalez


Spartina pectinata, also known as prairie cordgrass, has become a candidate for next generation biofuel research due to its ability to grow on marginal land and produce admirable levels of biomass. To aid in future genetic and breeding programs, a bacterial artificial chromosome (BAC) library, along with a protocol, has been constructed. With the genesis of this library, we were able to analyse 87 randomly picked clones to determine an average insert size of the library to be over 100 kbp in length. Along with the BAC library construction, ploidy level analysis was also performed via flow cytometry. This methodology allowed us to characterize the ploidy levels of our current prairie cordgrass population collection at South Dakota State University, which had an almost uniform ploidy level of 8x. A few of our samples from our collection also showed altering levels of ploidy which showed peak values that were either 4x, 6x, or even beyond that of what can be classified as 8x. The research done here has provided added insight into further developing prairie cordgrass as a next generation bio-fuel.

Library of Congress Subject Headings

Gene libraries


Includes bibliographical references



Number of Pages



South Dakota State University


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