Thesis - Open Access
Master of Science (MS)
Department / School
Apparently very little, if any, anatomical investigation has been made of the bulbil of Lilium tigrinum, though it has often been used as a propagating organ and consequently, the problem suggested attractive possibilities in an almost virgin field of investigation. Collections were made from five different plants beginning Sept. 27, 1928 until July 22, 1929. The older bulbils were removed from the axils of the foliage leaves and fixed in formalin acetic alcohol. They were gradually dehydrated by treatment with alcohol increasing the percentage 10% from 50 up to 100%. Xylol treatment in increasing percentages was then resorted to, until the bulbils could be exposed to absolute xylol without danger of excessive plasmolysis. The bulbils were embedded in paraffin and sectioned 10 microns thick with a rotary microtome. All the sections were mounted serially and stained in safranin; light green was used as a contrasting stain. The youngest bulbils were not removed from the plant stems at all. Together with the stem they were similarly fixed, embedded and out.
Library of Congress Subject Headings
Includes bibliographical references
Number of Pages
South Dakota State University
No Copyright - Non-Commercial Use Only
Tripp, Katherine Harris, "The Anatomy of the Germinating Bulbil of Lilium Tigrinum" (1930). Electronic Theses and Dissertations. 1913.