Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)




Mannitol, an alcohol of six carbon sugar-mannose, has a molecular weight of 182.17. It has the inability to enter the tricarboxylic acid cycle which makes mannitol a physiological stable compound. This chemical structure, physical and physiological properties of mannitol are listed in figure 1 Barry, (1963). The clinical use of mannitol as a therapeutic agent has been validated many times in the literature. The utilization of mannitol as a prophylactic measure in prevention of acute renal failure following surgery or trauma has been reported by many authorities. Before recently many surgeons have selected the utilization of mannitol rather than urea as a dehydrating fluid during neurosurgical operations Ohrt, (1967). There is limited and conflicting data describing mannitol's role in osmotic nephrosis of renal tissue. Available research employing experimental animals demonstrating the reversibility of these renal lesions in conjunction with mannitol infusion is very meager. The purpose of this study was to investigate physiological alterations and histological comparisons of mannitol in relation to proximal renal tubules employing virgin doe rabbits. Microscopic analysis was employed to determine reversibility of the histological changes altering tubule lumen size and distance of the nucleus from the basement membrane in respect to different dosage levels and various post-infusion sacrifice times.

Library of Congress Subject Headings





South Dakota State University