Thesis - Open Access
Master of Science (MS)
Dr. D.G. Kenefick
This research was initiated to obtain additional information about the causes for phenotypic variation of RNase I activity within winter barley cultivars. Unpublished data from this laboratory shows that antibody produced from RNase I obtained from two cultivars was mutually cross-reactive with antigen from the two cultivars of winter barley. The difference seen in RNase I activity between cultivars which can not be explained by the amount of RNase present may have similarities to that of inactive or incomplete forms of certain enzymes found in sec B mutants. Ferra-Novick et al. reported such sec B mutants as having early inactive forms of invertase, carboxypeptidase, and alpha-factor that accumulated due to incomplete penetration into the ER lumen. They also reported that these enzymes were not extractable by Triton X-100. Since antiRNase I was also cross-reactive with RNase II and was found to be a glycoprotein, it is proposed that RNase I is post-translationally modified to form RNase II; a process completed in the endomembrane system. It is also proposed that export of RNase occurs via the endoplasmic reticulum-Golgi complex. Based on these assumptions it was of interest to determine if applications of GA3 would cause differential secretion of RNase I or II from resistant and susceptible tissue, or somehow reflect different export programs, accounting for variation in enzyme content between the cultivars. The objectives of this study were: Objective 1: To evaluate the effect of exogenous GA3 on RNase in leaf tissue and the forms of RNase secreted from this tissue. Objective 2: To compare tissue levels and secreted amounts of RNase between a freeze-resistant and susceptible cultivar in response to an antagonist of endogenous GA3. Objective 3: To evaluate the effects of tissue desiccation on tissue ABA accumulation and RNase levels between a resistant and susceptible cultivar. Objective 4: To determine if alteration in RNase activity, resulting from changes in tissue ABA, would influence polynucleotide specificity.
Library of Congress Subject Headings
Barley -- Anatomy
Barley -- Frost resistance
South Dakota State University Theses
Number of Pages
South Dakota State University
Anderson, James Valentine, "Abscisic Acid and Tissue Desiccation Alters Ribonuclease I Activity in Winter Barley without Cold Acclimation" (1987). Electronic Theses and Dissertations. 4398.