Document Type

Thesis - Open Access

Award Date

1976

Degree Name

Master of Science (MS)

Department

Biology

Abstract

Variations in chromosome number and/or structure were reported to occur in long-term cultures (Heinz, Mee and Nickell, 1969; Heinz and Mee, 1971; Horak, Lustinec, Mesicek, Kaminek and Polackova, 1975; Kao, Miller, Gamborg, and Harvey, 1970; Nitra and Steward, 1961; Sacristan, 1971; Singh and Harvey, 1975a and b; Torrey, 1959). No change of chromosome number was found in the callus tissue or in the plants induced from the callu of Hemeocallis flava L. subcultured for six months (Chen and Holden, 1972). In this study, karyotype of the plants derived from a callus culture of H. flava, which had been subcultured for 30 months was analyzed to determine any change in chromosome structure. Meiotic studies of pollen- mother cells of the plants were also made to determine the nature of aberration in the chromosomes. Polyploid plants derived from a colchicine-treated long-term culture of the daylily callus were also investigated cytologically for obtaining indirectly the evidence of chromosome stability in the callus culture.

Library of Congress Subject Headings

Chromosomes

Plant tissue culture

Daylilies

Callus (Botany)

Format

application/pdf

Number of Pages

49

Publisher

South Dakota State University

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