Thesis - Open Access
Master of Science (MS)
Department / School
The objective of this research was to study the utilization of urea by mixed populations of rumen protozoa in vitro in the absence of bacteria. Initial experiments were conducted to determine the validity of chemical determinations used by other researchers in quantitating the nitrogen fractions in the medium following incubation. An experiment was conducted to determine the utilization of urea by rumen bacteria and this served as the basis for evaluating utilization of urea by protozoa. Culture techniques were developed to grow and maintain protozoa under in vitro conditions. Three experiments were conducted in which various nitrogen fractions of fermentation liquid were used as measures of urea utilization by rumen protozoa.
Library of Congress Subject Headings
Protozoology -- Research
Number of Pages
South Dakota State University
Vogel, Alan Lewis, "Metabolism of Urea Nitrogen by Rumen Ciliate Protozoa" (1971). Electronic Theses and Dissertations. 5264.