Document Type

Thesis - Open Access

Award Date

1971

Degree Name

Master of Science (MS)

Department

Animal Science

Abstract

The objective of this research was to study the utilization of urea by mixed populations of rumen protozoa in vitro in the absence of bacteria. Initial experiments were conducted to determine the validity of chemical determinations used by other researchers in quantitating the nitrogen fractions in the medium following incubation. An experiment was conducted to determine the utilization of urea by rumen bacteria and this served as the basis for evaluating utilization of urea by protozoa. Culture techniques were developed to grow and maintain protozoa under in vitro conditions. Three experiments were conducted in which various nitrogen fractions of fermentation liquid were used as measures of urea utilization by rumen protozoa.

Library of Congress Subject Headings

Protozoology -- Research

Nitrogen

Format

application/pdf

Number of Pages

77

Publisher

South Dakota State University

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