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Thesis - University Access Only
Master of Science (MS)
Biology and Microbiology
David A. Benfield
The virulent M-6 Miller and attenuated Purdue-115 strains of transmissible gastroenteritis virus (TGEV) were utilized to assess their binding properties to swine testis (ST) cell monolayers and identify a solubilized ST cell receptor. Attachment of TGEV to ST cells was determined by plaque assays and the binding of [35S]-methionine label led TGEV. It was found that the M-6 Miller strain of TGEV reached saturable binding sooner than the Purdue-115 strain of TGEV. The binding of both strains of TGEV was found to be dependent on the pH of the attachment medium (binding decreased at pH 5) and independent of cell age (4 versus 7 day old cells). Utilization of a direct virus binding assay (DVBA) revealed that both strains of TGEV bound to a 32-35 KDa ST cell membrane glycoprotein containing sialic acid. Binding was specific since it was inhibited by excess unlabelled TGEV. These data suggest that a membrane glycoprote in of 32 -35 kDA may be the putative receptor which mediates TGEV attachment to ST cells.
Library of Congress Subject Headings
Swine -- Virus diseases
Transmissible gastroenteritis of swine
Number of Pages
South Dakota State University
Flakus, Robert James, "Studies of Transmissible Gastroenteritis Virus Receptors on Swine Testis Cells" (1991). Electronic Theses and Dissertations. 5413.