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Document Type

Thesis - University Access Only

Award Date

1993

Degree Name

Master of Science (MS)

Department

Biology and Microbiology

First Advisor

R.N. Reese

Abstract

In soybean, more than half the flowers produce d may abort. Cytokinins have been found to be directly involved in floral development and in regulation of flower abortion. In the experimental soybean line IX93-100, many of the flowers that normally abort were induced to set by removal of proximal flowers and/or a single application of cytokinin. Differential expression of proteins in both setting and aborting tissues was examined by in situ radiolabeling of these proteins. These studies showed that induction of flower set resulted in a general increase in protein translation, concomitant with the differential expression of specific genes. Identification of unique genes specific for aborting or setting flowers was accomplished by use of Reverse Transcriptase and Polymerase Chain Reaction (PCR). The resulting (<1000pb) DNA polymers were resolved on a 6% DNA sequencing gel. Individual bands associated with aborting or setting tissues were excised from the gel and electroeluted. The specificity of expression will be verified using Northern blot analyses and the probes will be used to screen cDNA libraries. This method has the potential to allow rapid identification of genes regulating flower set and abortion.

Library of Congress Subject Headings

Soybean -- Genetics
Soybean -- Flowering

Format

application/pdf

Publisher

South Dakota State University

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