Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)


Biology and Microbiology

First Advisor

Jai S. Rohila


Pre-harvest sprouting (PHS) of wheat (Triticum aestivum L.) is a condition characterized by the early germination of spikes during moist environmental conditions. PHS lowers yield, degrades the quality of grain and thus limits the profits of wheat producers groups. During this investigation, proteomics studies of PHS-resistant and PHS-susceptible wheat embryos were conducted at different imbibition time periods via a cutting-edge technology called iTRAQ. Proteomic analysis revealed that 190 differentially expressed proteins might be involved in various cellular functions, such as carbohydrate metabolism, nitrogen metabolism, stress response, redox regulation, ATP synthesis, and protein translation, during this untimely germination of the wheat embryo. Hierarchical clustering analysis revealed the expression pattern of proteins in each of the resistant and susceptible germplasm and relative abundance of respective proteins between the two germplasm. Expression of stress-related and inhibitors proteins was found to be important in maintaining seed dormancy in resistant germplasm; whereas over-expression of energy metabolism related proteins was observed in PHS-susceptible germplasm for the production of energy required for seedling growth. ABA appeared to be involved in seed dormancy, directly or indirectly by controlling the expression of several LEA and EMB-1 proteins. A higher level of ROS production was observed in PHS-susceptible germplasm. Through bioinformatics analysis, a Thioredoxin h protein

Library of Congress Subject Headings

Wheat -- Preharvest sprouting,



Includes bibliographical references (page 78-102)



Number of Pages



South Dakota State University


Copyright © 2016 Pramod Khadka