Genetic Study of Aureobasidium pullulans Using Mutagenesis and Random Amplified Polymorphic DNA Analysis
Thesis - University Access Only
Master of Science (MS)
Department / School
Biology and Microbiology
Carl A. Westby
The objectives of this study were to: 1) Conduct mutagenesis using EMS and γ-irradiation to generate pullulan deficient (pul-) mutants from wild type A. pullulans ATCC 42023 (pul+). 2) Use Random Amplified Polymorphic DNA (RAPD) analysis to detect polymorphisms between A. pullulans ATCC 42023 and puI- mutants in order to generate possible probes for cloning pullulan biosynthesis related genes.
Library of Congress Subject Headings
Polysaccharides -- Synthesis
DNA -- Analysis
South Dakota State University
Zhang, Yong, "Genetic Study of Aureobasidium pullulans Using Mutagenesis and Random Amplified Polymorphic DNA Analysis" (1994). Electronic Theses and Dissertations. 20.