Thesis - University Access Only
Master of Science (MS)
Department / School
Biology and Microbiology
Cholinephosphotransferase is the enzyme which forms phosphatidylcholine, a major membrane lipid. The goal of this project was to produce a transgenic soybean which contained a modified cholinephosphotransferase gene in the antisense orientation. Antisense genes express the reverse complement of an mRNA. When 'this copy hybridizes with the normal mRNA it blocks translation, thereby eliminating the enzyme. In the case of cholinephosphotransferase this will eliminate the substrate for polyunsaturatec;I fatty acid synthesis, resulting in seeds with high levels of saturated and monounsaturated fatty acids. A particle inflow gun was used to target the anti-sense construction into soybean embryonic tissue. - The resulting tissue was regenerated. The antisense plasmid has been constructed, and has been successfully transformed into soybeans. However, the two soybeans that tested positive for the antisense construct could not be regenerated.
Library of Congress Subject Headings
Soybean -- Genetics
Number of Pages
South Dakota State University
Ellsworth, Buffy S., "Transformation of Soybean with an Antisense Cholinephosphotransferase Gene" (1997). Electronic Theses and Dissertations. 295.