Thesis - University Access Only
Master of Science (MS)
Department / School
Thomas P. West
In this study, Burkholderia cepacia ATCC 25416 was examined. This microorganism is considered to be clinically significant since it is an opportunistic human pathogen. Individuals with the genetic disorder cystic fibrosis are very susceptible to infection by B. cepacia. This study was undertaken to learn more about the pyrimidine biosynthetic pathway in this pathogenic pseudomonad. More specifically, it was of interest to learn whether the de nova pyrimidine biosynthetic pathway is controlled by pyrimidines at the level of gene expression. This was accomplished by using auxotrophic mutants of B. cepacia. The study of de nova pyrimidine biosynthesis in B. cepacia also allowed a comparison of the regulation of its de nova pyrimidine enzyme activities with what is known about the regulation of pyrimidine biosynthesis in similar microbial species.
Library of Congress Subject Headings
Pyrimidines -- Synthesis
South Dakota State University
Li, Ke, "Pyrimidine Biosynthesis in Burkholderia Cepacia" (1994). Electronic Theses and Dissertations. 66.