Document Type

Article

Publication Date

6-30-2016

Keywords

Adipokines, Bacteroides, Case-Control Studies, DNA, Bacterial, Diet, Eubacterium, Fatty Acids, Volatile, Feces, Gas Chromatography-Mass Spectrometry, Gastrointestinal Microbiome, Humans, Metabolic Syndrome, Principal Component Analysis, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Starch, Waist Circumference

Abstract

Dietary modulation of the gut microbiota impacts human health. Here we investigated the hitherto unknown effects of resistant starch type 4 (RS4) enriched diet on gut microbiota composition and short-chain fatty acid (SCFA) concentrations in parallel with host immunometabolic functions in twenty individuals with signs of metabolic syndrome (MetS). Cholesterols, fasting glucose, glycosylated haemoglobin, and proinflammatory markers in the blood as well as waist circumference and % body fat were lower post intervention in the RS4 group compared with the control group. 16S-rRNA gene sequencing revealed a differential abundance of 71 bacterial operational taxonomic units, including the enrichment of three Bacteroides species and one each of Parabacteroides, Oscillospira, Blautia, Ruminococcus, Eubacterium, and Christensenella species in the RS4 group. Gas chromatography-mass spectrometry revealed higher faecal SCFAs, including butyrate, propionate, valerate, isovalerate, and hexanoate after RS4-intake. Bivariate analyses showed RS4-specific associations of the gut microbiota with the host metabolic functions and SCFA levels. Here we show that dietary RS4 induced changes in the gut microbiota are linked to its biological activity in individuals with signs of MetS. These findings have potential implications for dietary guidelines in metabolic health management.

Publication Title

Scientific Reports

Volume

6

First Page

28797

Last Page

28797

Format

application/pdf

PMCID

PMC4928084

DOI of Published Version

10.1038/srep28797

Publisher

Nature Publishing

Rights

Copyright © 2016 the Author(s)

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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