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Mice are often the model of choice for genomic research because of their high similarity to the human genome and relatively easy maintenance. Thus, understanding the mechanisms and principles of their genome is vital to advancing our knowledge about human biology. Long interspersed elements type 1 (LINE-1s or L1s) are transposable elements within mammalian DNA. Promoter activities have been identified for the human L1 5’ untranslated region (5’UTR) in both the sense and antisense orientation, but it was previously unknown if mouse L1 5’UTRs have antisense promoter activity. The goal of my project was to uncover mouse L1 5’UTR promoter activity in the antisense orientation. Various mouse L1 5’UTR promoters were designed in the antisense orientation. The promoter constructs were transfected into NIH/3T3 mouse fibroblast cells, and the promoter activity was quantitatively assessed by a dual-luciferase assay. The results showed promoter activity in the antisense orientation. Like the human genome, mouse L1s display both sense and antisense promoter activities revealing a further similarity between the two species.




South Dakota State University


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