Document Type

Thesis - Open Access

Award Date

2019

Degree Name

Master of Science (MS)

Department / School

Biology and Microbiology

First Advisor

Yang Yen

Keywords

Chi-square test, GBS, Maize, Near isogenic lines, Perennialism, SNP

Abstract

Developing perennial grain crops is an effective way for sustainable agriculture and it can be a tool for the betterment of the overall ecosystem. To understand how perenniality works in perennial species, we made a cross between Zea diploperennis and maize (Z. mays) inbred line B73. F1 hybrids produced from this cross were selfed to get the F2 population and later the F2 population underwent Genotyping-By- Sequencing (GBS) analysis. Based on the candidate loci identified on chromosome 2 and chromosome 7 from GBS, SNP-specific markers were developed from the candidate locus interval and used to genotype the F2 population. X2 test for independence showed that the tested SNPs are associated with regrowth. Additionally, gene-specific markers (gt1, tb1, id1) were also used to genotype the F2 population and the X2test result indicates that none of these genes is significantly associated with regrowth. This result is inconsistent with the previous works conducted on these genes. Later the SNP-specific markers were used to select near-isogenic lines for the two regrowth loci.

Library of Congress Subject Headings

Corn -- Genetics.
Genetic markers.
Gene mapping.
Perennials.

Format

application/pdf

Number of Pages

84

Publisher

South Dakota State University

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Rights Statement

In Copyright