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Biological treatment methods are the basis for waste water treatment worldwide. Among biological treatment systems, activated sludge processes are most widely used to remove the oxygen demand and nutrients from wastewater. The activated sludge process uses naturally occurring bacteria at high concentrations in tanks. The bacteria in the activated sludge process remove small molecules of organic carbon, nitrogen, and phosphate as a consequence of bacterial growth and the waste water is purified. The effluent can then be safely released to the environment. Respirometry techniques are an effective tool to measure the respiration of living organisms. These techniques are associated with readily biodegradable organic carbon . Modern respirometry has the ability to automate data collection. Therefore, it is considered a simple and effective tool to measure the oxygen uptake or the rate of substrate depletion. The oxygen consumption is directly associated with substrate removal and biomass growth in respirometry. This makes it a useful technique for monitoring, modeling and controlling the activated sludge process.


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