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Document Type

Thesis - University Access Only

Award Date


Degree Name

Master of Science (MS)

Department / School

Chemistry and Biochemistry

First Advisor

Douglas Raynie


A method is developed to isolate and identify linalool from a genetically modified blue-green algae. The sample is mixed with octane in a 5:1 ratio and four milligrams of sodium chloride per milliliter of sample is added to the mixture. After phase separation, the organic layer is isolated and concentrated to one milliliter under nitrogen flow and analyzed using GC-MS. The lower and upper detection limits were found to be 0.1 x 10-6 mg/mL and 8 mg/mL respectively using this method. The calibration curve is linear from 0.1 x 10-6mg/mL to 8 mg/mL and the recovery of linalool from aqueous samples is found to be 83%.In a related project, fatty acids for hydrocarbon-fuel manufacturing were isolated from a freeze-dried algal species (Scenedesmus dimorphus). Algae is extracted using supercritical carbon dioxide (6000 psi, 100 ⁰C) and liquid carbon dioxide (2500 psi, room temperature) for about 45 minutes with 100 mL of liquid carbon dioxide into a vessel containing chloroform and methanol in equal amounts. The extract is dried under the stream of nitrogen to obtain a constant weight. The yield of fat components with supercritical extraction is compared with extraction using hexane, isopropyl alcohol or chloroform. The effect of cell pretreatment with bead beating or sonication is evaluated for the above extractions. The maximum yield of 11.8 %(w/w) is observed using isopropyl alcohol as a solvent and 3.02 %(w/w) with supercritical extraction respectively. Both pretreatment methods provided increased extraction yields. There is no significant change in the amino acid content of algae before and after carbon dioxide extraction.

Library of Congress Subject Headings

Biomass energy
Algae products


Includes bibliographical references (pages 33-36)



Number of Pages



South Dakota State University


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