Off-campus South Dakota State University users: To download campus access theses, please use the following link to log into our proxy server with your South Dakota State University ID and password.
Non-South Dakota State University users: Please talk to your librarian about requesting this thesis through interlibrary loan.
Thesis - University Access Only
Master of Science (MS)
Chemistry and Biochemistry
A method is developed to isolate and identify linalool from a genetically modified blue-green algae. The sample is mixed with octane in a 5:1 ratio and four milligrams of sodium chloride per milliliter of sample is added to the mixture. After phase separation, the organic layer is isolated and concentrated to one milliliter under nitrogen flow and analyzed using GC-MS. The lower and upper detection limits were found to be 0.1 x 10-6 mg/mL and 8 mg/mL respectively using this method. The calibration curve is linear from 0.1 x 10-6mg/mL to 8 mg/mL and the recovery of linalool from aqueous samples is found to be 83%.In a related project, fatty acids for hydrocarbon-fuel manufacturing were isolated from a freeze-dried algal species (Scenedesmus dimorphus). Algae is extracted using supercritical carbon dioxide (6000 psi, 100 ⁰C) and liquid carbon dioxide (2500 psi, room temperature) for about 45 minutes with 100 mL of liquid carbon dioxide into a vessel containing chloroform and methanol in equal amounts. The extract is dried under the stream of nitrogen to obtain a constant weight. The yield of fat components with supercritical extraction is compared with extraction using hexane, isopropyl alcohol or chloroform. The effect of cell pretreatment with bead beating or sonication is evaluated for the above extractions. The maximum yield of 11.8 %(w/w) is observed using isopropyl alcohol as a solvent and 3.02 %(w/w) with supercritical extraction respectively. Both pretreatment methods provided increased extraction yields. There is no significant change in the amino acid content of algae before and after carbon dioxide extraction.
Library of Congress Subject Headings
Includes bibliographical references (pages 33-36)
Number of Pages
South Dakota State University
In Copyright - Educational Use Permitted
Sakampally, Vara Prasad, "Extraction of Intermediates for the Manufacturing Biofuel from Algae" (2013). Electronic Theses and Dissertations. 1650.