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Document Type

Thesis - University Access Only

Award Date

2013

Degree Name

Master of Science (MS)

Department

Plant Science

First Advisor

Xing-You Gu

Abstract

Soybean, the second most-economically important crop in the U.S., was domesticated from its wild progenitor approximately 5,000 years ago. Over the millennia, cultivar development focusing on improving specific agronomic traits simultaneously decreased genetic diversity within domesticated populations. The homogeneity of the current cultivated germplasm prevents straightforward genetic analysis of agronomically important traits. To overcome this limitation, a population of recombinant inbred lines (RILs) was developed from a cross between a locallyadapted cultivar (Glycine max L. Merr.) and a wild line (G. soja Sieb and Zucc.) of soybean to map quantitative trait loci (QTL) associated with agronomically important traits considered to be domestication-related traits (DRTs). A total of 214 RILs were grown under field conditions in 2010 and 2011 and 11 DRTs were evaluated for individual plants from each RIL. Heritability estimates for these DRTs ranged from 0.89 for flowering time; 0.68 to 0.73 for leaflet length; 0.81 to 0.82 for leaflet width; 0.63 to 0.71 for leaflet length-to-width ratio; 0.80 for 100-seed weight; and 0.96 to 0.98 for seed coat pigments measured by reflecting spectra. A framework linkage map consisting of 152 simple sequence repeat (SSR) markers was constructed based on a subpopulation of 96 RILs. Ten QTL were identified in this subpopulation, including four for floral initiation, three for leaf dimensions, one for 100-seed weight, and two for seed coat color. Four of the 10 loci correspond to previously reported QTL for the same traits. The RIL materials developed for mapping the QTLs detected in this research are valuable to identify QTL for other agronomic traits or to clone genes underlying the DRT QTL.

Library of Congress Subject Headings

Soybean -- Genome mapping
Soybean -- Genetics

Description

Includes bibliographical references

Format

application/pdf

Number of Pages

92

Publisher

South Dakota State University

Rights

In Copyright - Non-Commercial Use Permitted
http://rightsstatements.org/vocab/InC-NC/1.0/

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