Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)

Department / School

Dairy Science

First Advisor

Chunyang Wang

Second Advisor

Srinivas Janaswamy


Anthocyanins function as antioxidants and offer numerous other health benefits, such as anti-atherogenic and anti-inflammation protection, and may aid in the prevention of degenerative diseases. Aronia berries, uniquely high in anthocyanins, have a great potential in improving human wellness. The objectives of this study were: (1) to develop a rapid and reproducible analytical method for estimating the anthocyanin amount in Aronia berries; and (2) to determine the effect of growing conditions on anthocyanin concentration. A standard curve method was established based on anthocyanin absorbance in the visible range. The results reveal that the developed protocol could yield rapid and reproducible values. Subsequently, a total of 51 Aronia samples of Viking, McKenzie, and Galicjanka varieties grown at nine locations (Ogle County, IL, Minnehaha County, SD, Harrison County, MO, Clay County, IA, Plymouth County, IA, Charles Mix County, SD, Buchanan County, IA, Webster County, IA, and Woodbury County, IA) in the Midwest region of the United States have been analyzed. In general, the anthocyanin amount increased throughout the harvest season. It is not statistically (p < 0.05) dependent on the mean maximum temperature, mean temperature, mean minimum temperature, precipitation, and latitude. However, berries grown in the southeastern locations tend to have higher anthocyanins than those from the northwestern region.

Library of Congress Subject Headings

Aronia -- Health aspects.


Includes bibliographical references (pages 66-71)



Number of Pages



South Dakota State University

Included in

Food Science Commons



Rights Statement

In Copyright