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Document Type

Thesis - University Access Only

Award Date

2016

Degree Name

Master of Science (MS)

Department

Chemistry and Biochemistry

First Advisor

Xiuqing Wang

Abstract

Porcine RNA viruses from the Nidovirales order, including porcine reproductive and respiratory syndrome (PRRSV) and porcine epidemic diarrhea virus (PEDV), have been causing great economic loss for the pork industry in the United States. Both PRRSV and PEDV cause increased mortality in suckling piglets. PRRSV, is a single-stranded positive sense RNA virus from the Arteriviridae family. It is postulated that the ability of nonstructural protein-1 alpha (Nsp1a) to form multimers plays a key role in viral mRNA synthesis and virus replication. Nsp1a contains a PXP domain which has been shown to be involved in the multimerization of human immunodeficiency virus Vif protein. Treatment of cells with synthetic peptide containing the PXP domain inhibited virus replication in vitro. We sought to determine whether synthetic peptide containing the PXP domain of PRRSV Nsp1a protein can inhibit virus replication. We observed that one PXP peptide treatment reduced the replication of PRRSV in MARC-145 cells, a susceptible cell line for PRRSV. We also observed a peptide dose dependent response in inhibiting virus replication. This indicates that peptide containing PXP domain may block Nsp1a multimerization and therefore reduce virus replication.

Library of Congress Subject Headings

Swine -- Virus diseases
Immune responses
Virus inhibitors
Nidoviruses
Porcine reproductive and respiratory syndrome
Peptide

Description

Includes bibliographical references

Format

application/pdf

Number of Pages

59

Publisher

South Dakota State University

Rights

In Copyright - Non-Commercial Use Permitted
http://rightsstatements.org/vocab/InC-NC/1.0/

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