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Document Type
Thesis - University Access Only
Award Date
1989
Degree Name
Master of Science (MS)
Department / School
Wildlife and Fisheries Science
First Advisor
Charles G. Scalet
Keywords
walleyes, growth, eggs, population, south dakota, habitat
Abstract
Triploid induction experiments were conducted on fertilized walleye (Stizostedion vitreum) eggs in 1987 and 1988. Different methods and different heat-shock treatment regimes were used between years; either the water temperature, initiation time, or the duration of the heat shock were varied. In 1987, three replicates of four treatments were conducted: 3SoC-10 min after fertilization (MAF)-1. 0 min, 3SoC-10 MAF-2.0 min, 400C-10 MAF-1.5 min, and 120C-10 MAF-1.5 min (control treatment). The heat-shock treatments of 3SoC-10 MAF-2.0min and 400C-10 MAF-1.5 min were lethal to walleye eggs acclimated to 120C. Total percent hatch for the treatments of 380C-lo MAF-1. O min and 12oC-10 MAF-1.5 min was estimated to be 37.7% and 36.9%, respectively. Walleye fry from these two treatments were stocked into 0.1 hectare hatchery rearing ponds in an attempt to rear them to the fingerling size. No walleyes survived to a size that would allow blood sampling, therefore no triploid assessment was made. In 1988, six heat-shock treatments and three control treatments were conducted The water temperature of the heat shocks was 29oC or 31 CC, the initiation times were either 1, 3, or 5 MAF, and all heat shocks 1mted for 25 min. Control treatmen1s had the same initiation time and duration regimes, but the water temperature was I
Library of Congress Subject Headings
Walleye (Fish) -- Eggs
Walleye (Fish) -- Growth
Walleye (Fish) -- Identification
Flow cytometry
Description
Includes bibliographical references (page 59-66)
Format
application/pdf
Number of Pages
79
Publisher
South Dakota State University
Rights
Copyright © 1989 Rebecca Ewing
Recommended Citation
Ewing, Rebecca R., "Production and Flow Cytometric Identification of Triploid Walleyes" (1989). Electronic Theses and Dissertations. 347.
https://openprairie.sdstate.edu/etd/347