Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)

Department / School



The history of plant tissue culture dates back to the early 1900’s with the research of Gottleib Haberlandt in Germany. However, it was not until 1939 that any real success was achieved. Three independent scientists, Gautberet, Nobecourt, and White simultaneously through their work set the pace for plant tissue culture. Plant tissue culture is the isolation of cell or tissues from their association with other cells and tissues in the plant to one in which nutrients are supplied artificially and the cell or tissue acts as an individual. Once the plant cells or tissues have been studied at the individual level, more information about plant functions can be obtained. Most tissue culture research has involved cells or tissues taken from many species of dicotyledonous plants but from very few monocotyledons. This thesis reports an attempt to initiate and grow callus tissue of the monocotyledon, sorghum. The objectives of the research were the following: to determine the conditions necessary to produce callus; to describe the morphology of callus origin and development; and to reproduce the results obtained with dicotyledons, the totipotency of cells (Sun 1966). To the author's knowledge, no research to date has been published on the tissue culture of sorghum.

Library of Congress Subject Headings

Tissue culture



Number of Pages



South Dakota State University