Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)




The name "epizootic hemorrhagic disease" (EHD) of deer was first given by Shope et al. in 1955 because of the occurrence of the hemorrhagic disease in large epizootic_ proportions and because of its pathological features. It was also determined by them that the causative agent for the disease is a virus. Difficulties in studying the causative agent have been encountered because no host system other than the deer itself was available to support replication or detect the virus. However, isolation of the New Jersey strain of EHD virus was accomplished by Mettler et al. in 1962 using newborn mice and Hela cells. Limited success of the isolation of South Dakota strains of EHD virus was achieved by Pirtle and Layton in 1961. Yet there is no one ideal tissue culture system that has been applied to the study of EHD virus, especially the South Dakota strain. Therefore, there is a need for a cell culture system in which the cytopathogenic effect is obvious, rapid in onset, regularly reproducible, and.the yield of virus is high. This study was undertaken to develop a tissue culture system which would allow the detection, isolation and characterization of the virus. In order to develop a rapid laboratory diagnostic method for the disease, the study was extended to determine the relationship between the EHD disease and the total serum cholesterol level.

Library of Congress Subject Headings

Communicable diseases in animals


South Dakota State University Theses



Number of Pages



South Dakota State University