Carla M. Cass

Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)

Department / School

Biology and Microbiology

First Advisor

W. Kennedy Gauger


Cellulosic wastes are the most abundant organic material on earth. Utilization of cellulose occurs through microorganisms by a process of hydrolysis to low molecular weight hexoses and pentoses. These are regarded as substrates for microbial production of food (single cell protein), ethanol (fuel alcohol) butanol and other fermentation chemicals. Agricultural producers incur the largest volumes of these cellulosic wastes, therefore it is of economical importance that they be utilized. However, the owner-operator system for the utilization of in-situ produced cellulose for conversion to glucose has yet to be totally resolved. Since the facility at SDSU is similar to that of an individual owner-operator, this study was organized to determine how effective scale-up from a laboratory scale production level to a rudimentary pilot plant would be. The second objective was to determine if a simpler enzyme assay could be developed to replace the commonly used assays which are tine and labor intensive. The method chosen was Pharnacia Fine Chemicals chrormatofocusing which separates proteins by their respective isoelectric points. Thus, the enzyme complex can be analyzed for completeness.

Library of Congress Subject Headings

Enzymatic analysis



Number of Pages



South Dakota State University


No Copyright - United State