Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)

Department / School

Home Economics

First Advisor

Roger A. Shewmaker


The effects of the diet suggested in the United States Dietary Goals on serum cholesterol levels was studied by using male Holtzman rats. There were twelve experimental diets. All of the diets contained 28% of total calories as fat. The type of fat was either saturated, unsaturated, or half saturated and half unsaturated. Two sources of carbohydrate, sucrose and cornstarch, and tw6 levels of each, 52% and 62%, were examined. The source of protein was the same in all diets, but varied in amount, 10% or 20% of total calories. The type of fat did not yield a significant difference in total cholesterol, but HDL cholesterol levels were significantly higher in diets with unsaturated fat. Diets containing sucrose yielded significantly higher total cholesterol levels than cornstarch based diets, especially when the fat was half saturated and half unsaturated. Percent HDL cholesterol levels were significantly higher in the starch diets than in the sucrose diets. Level of protein in the diets produced no significant effects except that total cholesterol and percent HDL cholesterol were highest when the lowest level of protein was combined with saturated fat. As the level 6f sucrose increased and levels of protein decreased, total cholesterol increased and percent HDL cholesterol decreased. The effects were just the opposite with starch, total cholesterol decreased and percent HDL increased. From these results, it appears that the source and level of carbohydrate in the diet had the most effect on serum cholesterol levels.

Library of Congress Subject Headings

Blood cholesterol
Nutrition policy -- United States
Food -- Cholesterol content.



Number of Pages



South Dakota State University


No Copyright - United State