Thesis - Open Access
Master of Science (MS)
Department / School
In order to determine environmental effects upon different oat varieties, Benson, Chief, Froker, Lancer, Noble, and Spear were grown under greenhouse and field conditions. Lancer and Noble were crossed in the greenhouse to determine the genetic makeup of the fluorescent characteristic. Subsequent F3 generation seed was grown out and observed under greenhouse conditions alongside check samples of Noble and Lancer oat varieties. The early oat selections tested were all new entries and were grown at eleven locations in the United States. The midseason selections used were also new entries and were grown at twelve U.S. locations and Ottawa, Canada. New entries were chosen to insure cultivar uniformity and purity. New entries made this possible by supplying seed originating from one seed source. Cooperators growing early and midseason nurseries were requested to send 100 grams of clean seed after harvesting had been completed. A total of 279 samples of early and midseason oats were received. Each of these samples were divided into four equal sized samples of twenty-five grams, observed under ultraviolet light, and separated according to fluorescent characteristics. A vibrating seed tray with overhead fluorescent light was used to aid in separating the fluorescent and non-fluorescent seeds. In order to minimize judgmental errors, all samples of a particular entry were tested and completed as a set. The same ultraviolet light, a standard tube emitting 3600 angstrom units, was used for all samples tested. Background consisted of white contact paper on a plastic tray which is standard on the vibrating seed tray used at South Dakota State University Seed Testing Laboratory. The oat fluorescence test has been conducted on a weight percentage basis and off-types per pound basis, with mixed results. Additional factors were measured and analyzed in this study to help determine the accuracy of the oat fluorescence test, as a means of detecting varietal purity. Factors used were maturity, location, variety, seeds per twenty-five grams, fluorescent seeds per twenty-five grams, non-fluorescent seeds per twenty-five grams, yield per acre, test weight per bushel, longitude, latitude, altitude, moisture conditions, and temperature conditions. In addition to the tests conducted on the early and midseason oat cultivars, subsequent tests were conducted on bulked seed of segregating F3 generations. Eight midseason samples from two locations, Brookings and Watertown, SD, were used to determine if the model developed from previous findings would be effective in detecting mixtures.
Library of Congress Subject Headings
Oats -- Varieties
Oats -- Seed
South Dakota State University Theses
Number of Pages
South Dakota State University
Van de Crommert, John, "Fluorescence in Oats and its Value as a Varietal Purity Indicator" (1983). Electronic Theses and Dissertations. 4327.