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Document Type

Thesis - University Access Only

Award Date


Degree Name

Master of Science (MS)



First Advisor

Timothy J. Gutormson


Accelerated aging germination is a method of predicting field emergence of soybean seed under unfavorable growing conditions. This test accelerates normal seed deterioration by placing seed under 41°C and 95-100% relative humidity conditions for 72 hours prior to germination testing. Germination decline following accelerated aging is proportional to initial seed physiological potential. Variability within and between chamber types has resulted in significant variation among results as detected by Association of Analysts (AOSA) vigor testing referees. laboratory test Official Seed Condensation collecting inside the chamber is commonly cited as causing inconsistency of test results. Water dripping from the chamber roof onto sample box lids causes condensation within boxes resulting in excessively high final seed moisture. Water jacketed incubators designed for tissue cell culture growth prevent water condensation by exposing interior surfaces to a constant temperature maintained by the water jacket. Objectives of this research were to evaluate a water jacketed incubator (NuAire NU-1700) by (1) determining appropriate box type and seed sample weight for NU-1700 aging, (2) checking NU-1700 uniformity, and (3) measuring relationship of NU-1700 accelerated aging results with field emergence.

Library of Congress Subject Headings

Soybean -- Seeds -- Testing

Soybean -- Seeds -- Viability



Number of Pages



South Dakota State University