Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)

Department / School



South Dakota 10 (SD 10) virus strain of epizootic, hemorrhagic disease (EHD). of deer has not been compared chemically and morphologically to the reference strain of EHD, New Jersey, .and other strains. In addition, the question has arisen if EHD viruses may be the same viruses as the bluetongue viruses found in sheep and cattle. Therefore, an investigation was undertaken to characterize SD 10 virus in comparison to other EHD virus strains with Bluetongue 262, Reovirus 3, Infectious Bovine Rhinotracheitus virus, and Enteric Cytopathic Bovine Orphan virus as control viruses. All viruses used in this study were adapted to African Vero Green Monkey Kidney (Vero) cells. The virus effects on the tissue culture cells were studied. Hemagglutination and serum neutralization tests were used to show antigenic characteristics and relationships between viruses. Various tissue culture staining techniques were used to help determine the nucleic acid content of the virus induced intracytoplasmic inclusions. Enzyme digestion, in addition to chemical and metabolic inhibitors, was used to help identify the nucleic acid. Electron microscopy was used to study the viruses in mouse brain and. Vero cells and free SD 10 virus stained negatively. Characteristics of the virus infection and the size of the viruses were determined by examination of the thin sections. The negative stain was used to count the capsomere on the capsid or coat of SD 10 virus.

Library of Congress Subject Headings

Virology -- Research



Number of Pages



South Dakota State University