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Qi Sun

Document Type

Thesis - University Access Only

Award Date


Degree Name

Master of Science (MS)

Department / School


First Advisor

Duane Matthees


Regulatory analysis of commercial products is an important consumer service. It is necessary to compare the indicated level of penicillin G with the actual level in feed samples. The analysis of feed samples assures the farmer that he will be receiving a fair value for his money. The ideal method would be one which is accurate, fast and precise. A great variety of chemically based techniques for the analysis of penicillin G have been reported, including iodine titration, hydroxylamine colorimetry, gas chromatography, thin-layer chromatography, and high-performance liquid chromatography (HPLC). However, most of these methods were developed for analysis bf relatively pure products or of higher levels and have not previously been reported for feed analysis. Several early methods for the estimation of penicillin by direct measurement of UV absorbance were reported(29), but these methods were found to be mainly applicable to reasonably pure samples of penicillin. In order to enhance selectivity and sensitivity, several high-performance liquid chromatographic(HPLC) methods combined with precolumn and postcolumn reactions have been developed for the assay of penicillin. The purpose of this study was to establish a rapid and selective method for the determination of penicillin G in animal feed by combining a simple pre-column derivatization treatment with HPLC separation.

Library of Congress Subject Headings

Feeds -- Analysis
High performance liquid chromatography



Number of Pages



South Dakota State University