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Document Type

Thesis - University Access Only

Award Date


Degree Name

Master of Science (MS)

Department / School


First Advisor

C.H. Chen


Anther culture is a tool for the production of haploid (n) and double haploid (2n) plants, which are of great theoretic value to wheat breeders for improving the efficiency of selection in relatively small populations. Practical application of double haploid breeding in wheat was limited due to low frequency of double haploid plant production, especially using elite germplasm. Although environmental effects on pre- and post-inoculation of anther were noted by Lazar et al. (1984) and Jones et al. (1987) , anther culture responses in wheat have generally been more dependent upon genotype than culture conditions (Bullock et al., 1982; Liang et al., 1987). Ouyang et al. (1983) and Buyser et al. (1987) reported high frequencies in polyhaploid callus and plant production in anther cultures of certain wheat genotypes. The objectives of this study were: (1) to test for the green plant regenerability, and (2) to assess the feasibility of isolating true-breeding segregates from anther culture of South Dakota spring wheat breeding stock of F1 hybrids with the ultimate goal of immediate genetic fixation of desirable economic traits into white-seeded lines.

Library of Congress Subject Headings

Wheat -- Genetics
Wheat -- Breeding
Wheat -- Cultures and culture media



Number of Pages



South Dakota State University