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Document Type

Thesis - University Access Only

Award Date

1991

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Michael B. Hildreth

Abstract

The main purpose of the project included in this thesis was to evaluate the feasibility of developing a diagnostic technique utilizing lectins to differentiate the eggs of E. multilocularis from those of other dog taeniids such as T. pisiformis. This evaluation included an attempt to identify one or more lectins that bound to one or more of the egg membranes of E. multilocularis or T. pisiformis, but not both. Initially, the evaluation was conducted on plastic embedded sections of whole eggs of E. multilocularis, T. pisiformis, and T. taeniaeformis. Sections were used so that the binding to each structure could be observed simultaneously. This provided much information on the carbohydrate composition of the taeniid egg and provided direction for further research on whole eggs. Lectin binding to the embryophore and oncospheral membrane of whole, fixed eggs was then observed for each of the three species. Access to the oncospheral membrane was gained by removal of the embryophore (hatching) using sodium hypochlorite (NaOCl). The final portion of this research was conducted in order to assess the value of NaOCI as a method of hatching eggs for this lectin technique.

Library of Congress Subject Headings

Echinococcus
Dogs -- Parasites
Dogs as carriers of disease
Echinococcosis -- Diagnosis

Format

application/pdf

Publisher

South Dakota State University

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