Dissertation - Open Access
Doctor of Philosophy (PhD)
Agronomy, Horticulture, and Plant Science
Jose L. Gonzalez Hernandez
Sunish Kumar Sehgal
Triticum aestivum, wheat, Stagonospora nodorum blotch, SNB, GWAS, QTL, HWWAMP, SNP markers.
Stagonospora nodorum blotch (SNB) is an economically important wheat disease caused by the necrotrophic fungus Parastagonospora nodorum. SNB resistance in wheat is controlled by several quantitative trait loci (QTLs). Thus, the identifying of novel resistance/susceptibility QTLs is crucial for continuous improvement of the SNB resistance. Here, the hard winter wheat association mapping panel (HWWAMP) comprising accessions from breeding programs in the Great Plains region of the US, was evaluated for SNB resistance and necrotrophic effectors (NEs) sensitivity at the seedling stage. A genome-wide association study (GWAS) was performed to identify singlenucleotide polymorphism (SNP) markers associated with SNB resistance and effectors sensitivity. We found seven significant associations for SNB resistance/ susceptibility distributed over chromosomes 1B, 2AL, 2DS, 4AL, 5BL, 6BS, and 7AL. Two new QTLs for SNB resistance/ susceptibility at the seedling stage were identified on chromosomes 6BS and 7AL, whereas five QTLs previously reported in diverse germplasms were validated. Allele stacking analysis at seven QTLs explained the additive and complex nature of SNB resistance. We identified accessions (‘Pioneer-2180’ and ‘Shocker’) with favorable alleles at five of the seven identified loci, exhibiting a high level of resistance against SNB. Further, GWAS for sensitivity to NEs uncovered significant associations for SnToxA and SnTox3, co-locating with previously identified host sensitivity genes (Tsn1 and Snn3). Candidate gene analysis for SNB resistance revealed 35 genes of putative interest with plant defense response-related functions. The QTLs identified and validated in this study could be easily employed in breeding programs using the associated markers to enhance the SNB resistance in hard winter wheat.
Library of Congress Subject Headings
Winter wheat -- Disease and pest resistance -- Genetic aspects.
Fungal diseases of plants.
Wheat -- Diseases and pests.
Number of Pages
South Dakota State University
Copyright © 2021 Rami Altameemi
Altameemi, Rami, "Genome-wide Association Analysis Permits Characterization of Stagonospora nodorum blotch (SNB) Resistance in Hard Winter Wheat" (2021). Electronic Theses and Dissertations. 5491.