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Document Type

Thesis - University Access Only

Award Date

1992

Degree Name

Master of Science (MS)

Department

Biology and Microbiology

First Advisor

Nels H. Granholm

Abstract

Specific agouti gene products may modulate the activity of tyrosinase in several possible ways. These include gene activation or deactivation, differential transcription, translation, post-translational events such as glycosylation, removal or addition of inhibitors or activators of tyrosinase, and other specific tissue-dictated events. The purpose of this study was to test the hypothesis that a/a and AY/a melanocytes exhibit different rates of tyrosinase synthesis. By incubating skins or harvested hairbulbs in the presence of 35S-methion1ne, newly translated protein would contain radio-labelled amino acid. Using tyrosinase-specific antibody raised against mouse melanoma tyrosinase in rabbits to immunoprecipitate radio-labelled tyrosinase, it is possible to detect differential translation rates between various agouti genotypes. Control samples were treated in exactly the same fashion as experimentals, except normal rabbit serum was used in place of anti-tyrosinase antibody. The complete experimental protocol is presented in the Materials and Methods section.

Library of Congress Subject Headings

Mice -- Genetics
Melanocytes
Pigments (Biology)
Melanins -- Synthesis

Format

application/pdf

Publisher

South Dakota State University

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