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Faculty Mentor

Marie-Laure Sauer, Fedora Sutton

Abstract

Non-radioactive isotopic labeling has become a regular technique for efficiently labeling a wide range of macromolecules. The overall goal for this project was to develop a method of globally measuring plant protein turnover rates. In order to do so, an isotopic labeled environment that does not induce stress had to be used. The objective of this particular segment of research was to develop a 15N-labeled Tris-Acetate-Phosphate (TAP) media in which Chlamydomonas reinhardtii can successfully grow without eliciting stressed physiological responses. Our results illustrate that we have successfully developed 15N-labeled TAP that does not stress Chlamydomonas reinhardtii.

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