Time and Temperature Requirements for Effective Removal of High Molecular Weight RNA from Winter Wheat Genomic DNA with LiCl

Authors

Benjamin Meyerink, South Dakota State University
Ryan Geraets, South Dakota State University

Faculty Mentor

Fedora Sutton

Abstract

Numerous protocols are available for the isolation of plant genomic DNA. Often times, these protocols utilize a wide variety of solutions. The primary purpose of this project was to examine the use of LiCl for the removal of RNA contaminants within genomic DNA samples and its dependency on incubation time and temperature. Our results indicate that LiCl is sufficient for the removal of high molecular weight RNA contaminants from genomic DNA. In addition, our results illustrate varying incubation times with LiCl yield minimal differences in the recovery of genomic DNA and the removal of RNA contaminants. Alternatively, different incubation temperatures produce greater variation in the recovery of genomic DNA and the removal of RNA contaminants. The genomic DNA that was extracted using the appropriate incubation requirements was examined for possible use in downstream applications via polymerase chain reaction (PCR).

Recommended Citation

Meyerink, Benjamin and Geraets, Ryan (2009) "Time and Temperature Requirements for Effective Removal of High Molecular Weight RNA from Winter Wheat Genomic DNA with LiCl," The Journal of Undergraduate Research: Vol. 7, Article 6.
Available at: https://openprairie.sdstate.edu/jur/vol7/iss1/6