Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)

Department / School

Animal Science


Because of the time consuming and costly nature of the total collection in vivo digestion trials, various other methods have been tried to get reliable digestibility data in a faster and less costly manner. One of the more recent methods being used is the “artificial rumen” (in vitro) technique. This method makes use of rumen bacteria and simulated conditions in the rumen of the animal. Cost, tie, and labor saving are definitely in its favor. Some of the work reported indicates the in vitro technique can be a useful method in nutrition research. Studies on the effect of stage of maturity when harvested on digestibility of forages and the effect of various feed additives on digestibly of roughages have made use of the in vitro digestion technique. However, its accuracy and conditions for operation need further testing. In order for the artificial rumen technique to be used extensively in digestion studies, the results obtained would be comparable to results obtained with the animals. Therefore, the work reported in this thesis was designed to make a series of comparisons of cellulose digestion between the in vivo and in vitro methods.

Library of Congress Subject Headings

Rumination (Digestion)


Includes bibliographical references



Number of Pages



South Dakota State University