Document Type
Thesis - Open Access
Award Date
1988
Degree Name
Master of Science (MS)
Department / School
Chemistry
First Advisor
Duane P. Matthees
Abstract
Mycophenolic acid is toxic to animals consuming it beyond a certain level and possesses antitumor activity. Not many methods for its trace analysis in feedstuffs and agricultural products have been reported. Thus, it is desirable to develop a standard analytical technique for such important compounds. The purpose of this investigation is to develop a technique or method sensitive enough to determine trace quantities of the compound. The method developed is based on solvent extraction from feedstuffs and detection by high performance liquid chromatography. Solvent extraction methods have given good results with low background interferences when cleaned up by column chromatography and/or acid base partitioning (for some grain samples only). High performance liquid chromatography was chosen for the detection of mycophenolic acid because it offers a high degree of sensitivity and selectivity with very few background interferences when compared to gas chromatography.
Library of Congress Subject Headings
Feeds -- Contamination
Feeds -- Analysis
Chromatographic analysis
Format
application/pdf
Number of Pages
59
Publisher
South Dakota State University
Recommended Citation
Kahsai, Tesfamichael Zeccarias, "Development of an Analytical Method for the Trace Determination of Mycophenolic Acid by High Performance Liquid Chromatography Following Extraction of the Compound from Feedstuffs" (1988). Electronic Theses and Dissertations. 4513.
https://openprairie.sdstate.edu/etd/4513