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Document Type

Thesis - University Access Only

Award Date

1989

Degree Name

Master of Science (MS)

Department / School

Animal Science

First Advisor

Douglas C. McFarland

Abstract

Proliferation and differentiation (fusion) of myogenic satellite cells derived from Nicholas tom and hen turkeys at 3, 9, and 15 weeks of age, and the response of a satellite cell clone to serum from these birds was examined. Satellite cells were isolated from pooled samples of pectoralis major (PM) muscles from birds in each age and sex group, and anterior latissimus dorsi (ALD) muscles of 15-week-old toms. In primary and tertiary cultures grown in McCoy's SA medium containing 15% chicken serum for 120 or 72 h, respectively, relative cell proliferation increased (P < .05) with donor age. Sex of bird had no effect on satellite cell proliferation in tertiary cultures after 72 h (P> .17). Fusion percentages averaged 81.0 + 0.7% following 72 h exposure to Dulbecco's Modified Eagle's Medium containing 1% horse serum. Turkey serum (TS) samples were processed for each bird individually (n=27), and the effect of serum source on cell growth was measured using a satellite cell clone bioassay. Half-maximal stimulation of growth occurred with McCoy's SA medium containing 3% TS. When turkey satellite cell clones were exposed to medium containing 3% serum from birds in the various groups, an age-related decline in proliferation occurred with serum from hen but not tom turkeys (P.05). iv Cells derived from PM and ALD muscles possessed similar proliferation and fusion properties in vitro. A flow cytometric method which may be used to evaluate the effects of various agents on the satellite cell-cell cycle was also described. The results suggest that (1) satellite cells of growing turkeys retain a high proliferation potential, (2) the mitogenic activity of turkey serum corresponds with growth rate of the donor, and (3) satellite cells isolated from PM and ALD muscles respond similarly to culture conditions.

Library of Congress Subject Headings

Turkeys -- Growth

Satellite cells

Muscle cells

Format

application/pdf

Number of Pages

77

Publisher

South Dakota State University

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