In Vitro Analysis of Pre- and Early Postimplantation Development of Lethal Yellow (Ay/Ay) Mouse Embryos

Author

Linda L. Johnson

Document Type

Thesis - Open Access

Award Date

1977

Degree Name

Master of Science (MS)

Department / School

Biology

Abstract

The dominant A^Y gene of the agouti locus (linkage group V) in the house mouse (Muse musculus) is one of approximately eleven alleles specifying coat color. Homozygosity of the A^Y allele is thought to cause death of the embryo in utero around the time of implantation. For this reason the A^Y/A^Y genotype is called the lethal yellow mouse. The precise time that the primary gene defect is first expressed is not known. Attempts to pinpoint the phenocritical period in an effort to discover the basic biochemical alteration specified by the lethal gene have thus far be unsuccessful. An in vitro analysis was therefore undertaken to meet the following objectives: 1. Define morphological characteristics that will identify the class of A^Y/A^Y homozygotes in preimplantation embryo culture, 2. Discover the extent of the phenocritical period of A^Y homozygotes, 3. Discover if A^Y homozygotes are in fact implantation lethals and 4. Test the background variability and abnormalities occurring in control crosses as compared to the experimental cross.

Library of Congress Subject Headings

Embryology

Ovum implantation

Mice

Format

application/pdf

Number of Pages

66

Publisher

South Dakota State University

Recommended Citation

Johnson, Linda L., "In Vitro Analysis of Pre- and Early Postimplantation Development of Lethal Yellow (Ay/Ay) Mouse Embryos" (1977). Electronic Theses and Dissertations. 5080.
https://openprairie.sdstate.edu/etd/5080