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Document Type

Thesis - University Access Only

Award Date

1991

Degree Name

Master of Science (MS)

Department / School

Biology and Microbiology

First Advisor

David A. Benfield

Abstract

The virulent M-6 Miller and attenuated Purdue-115 strains of transmissible gastroenteritis virus (TGEV) were utilized to assess their binding properties to swine testis (ST) cell monolayers and identify a solubilized ST cell receptor. Attachment of TGEV to ST cells was determined by plaque assays and the binding of [35S]-methionine label led TGEV. It was found that the M-6 Miller strain of TGEV reached saturable binding sooner than the Purdue-115 strain of TGEV. The binding of both strains of TGEV was found to be dependent on the pH of the attachment medium (binding decreased at pH 5) and independent of cell age (4 versus 7 day old cells). Utilization of a direct virus binding assay (DVBA) revealed that both strains of TGEV bound to a 32-35 KDa ST cell membrane glycoprotein containing sialic acid. Binding was specific since it was inhibited by excess unlabelled TGEV. These data suggest that a membrane glycoprote in of 32 -35 kDA may be the putative receptor which mediates TGEV attachment to ST cells.

Library of Congress Subject Headings

Swine -- Virus diseases
Transmissible gastroenteritis of swine

Format

application/pdf

Number of Pages

79

Publisher

South Dakota State University

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