Document Type
Thesis - University Access Only
Award Date
1995
Degree Name
Master of Science (MS)
Department / School
Chemistry and Biochemistry
First Advisor
Donald P. Evenson
Abstract
In the first study, to determine the effects of iii elevated temperatures on germ cells, scrotal regions of mice were exposed to 38°, 40° or 42°C for one hour. On days 1, 3, 7, 11, 14, 21, 28, and 35 post-exposure, mice were killed and testis weights recorded. Testicular cell populations were analyzed using acridine orange staining in conjunction with flow cytometry. The sperm chromatin structure assay which measures the susceptibility of sperm DNA to in situ acid denaturation was performed on cauda epididymal cells. Mice exposed to 42°C showed the largest changes in testicular weights and cell ratios. Testicular weights were significantly lower days 1 (p
Library of Congress Subject Headings
Mice -- Genetics
Germ cells
Chromatin
Flow cytometry
Format
application/pdf
Publisher
South Dakota State University
Recommended Citation
Sarkar, Linda Jean, "Flow Cytometric Examinations of Mouse Germ Cell Chromatin" (1995). Electronic Theses and Dissertations. 147.
https://openprairie.sdstate.edu/etd2/147
Rights Statement
