Document Type

Thesis - University Access Only

Award Date


Degree Name

Master of Science (MS)

Department / School

Biology and Microbiology


Twelve bovine viral diarrhea virus (BVDV) field isolates and reference strains were evaluated for their ability to inhibit T and B cell mitogenesis in vitro. These twelve field and reference strains represent four groups of BVDV divided by the combination of genotype and cytopathic character. T lymphocyte proliferation was inhibited by a majority of the twelve field and reference strains. Field isolate #17 and reference strains 125 and NADL induced 40-70 percent inhibition of T lymphocyte mitogenesis. I n contrast, strain 890 and field isolate #12508 showed no inhibitory effects on T lymphocyte mitogenesis. The remaining strains and field isolates routinely inhibited T lymphocyte proliferation by 4-40%. B lymphocyte proliferation, stimulated by PWM, was inhibited by only seven out of twelve BVDV strains. However all of the strains, except N. Y. I, inhibited B lymphocyte proliferation stimulated by STM. No strain consistently demonstrated weak or strong inhibition of both PWM·and STM stimulated B lymphocytes. The mitogenic combination, ION + PMA, was not significantly inhibited by any of the BVDV strains tested. The co-mitogen combination induces proliferation of T lymphocytes through direct interactions with the signal transduction cascade. All of the other mitogens induce proliferation by interaction with cell membrane associated receptors. Thus, this difference in inhibition suggests the probable location within the signal transduction pathway that BVDV inhibits lymphocyte mitogenesis. There was no significant or reproducible pattern of inhibition of T or B lymphocyte mitogenesis when analyzed by genotype or biotype. Also, no significant difference between field isolates and characterized reference strains were observed. Thus, the differences observed in the study appeared to be unique to the individual viruses.

Library of Congress Subject Headings

Bovine viral diarrhea


T cells

B cells



Number of Pages



South Dakota State University