Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)

Department / School

Biology and Microbiology

First Advisor

Nicholas Butzin


The theme of this thesis revolves around how bacteria respond and thrive during stress. Chapters 1-3 are about how bacteria deal with life-threatening antibiotics. Chapter 4 covers new research on how bacteria can move from a stressful individual lifestyle (free-living bacteria) to initiating a symbiotic relationship with a plant (a less stressful lifestyle). In Chapter 1, I briefly summarize the current state of knowledge in the field of antibiotic resistance and persistence. In Chapter 2, I add to this knowledge by providing new insights into several antibiotics' potency and exploring the antibiotic Eagle effect. In Chapter 3, I use pyruvate to study the revival of persister cells. Finally, in Chapter 4, I switch gears and briefly discuss how I optimized the initial steps of soybean germination and rhizobia culturing techniques to monitor the root-bacterial attachment.

Library of Congress Subject Headings

Escherichia coli.
Escherichia coli -- Effect of drugs on.
Soybean -- Roots.
Drug tolerance.
Drug resistance.

Number of Pages



South Dakota State University



Rights Statement

In Copyright