Document Type

Thesis - Open Access

Award Date


Degree Name

Master of Science (MS)

Department / School

Biology and Microbiology

First Advisor

Madhav Nepal


WRKY transcription factors (TFs) are DNA-binding proteins, which play important roles in managing plant stresses and regulating growth and development processes. The WRKY TF family has expanded significantly through genome/gene duplications in the plant evolutionary process. Although the WRKY TFs have been identified in hundreds of plant species, they are yet to be identified in Amborella trichopoda, a species that represents the most basal group of Angiosperms. The main objectives of this study were to conduct genome-wide identification of the WRKY gene family in A. trichopoda and to assess their homologs in Angiosperms. In this study, we identified 42 A. trichopoda WRKY TFs and, and their homologss in seven other plant species (Cinnamonum micranthum, Asparagus officinilas, Glycine max, Gossyopium raimondii, Arabidopsis thaliana, Chenopodium quinoa, and Helianthus annuus). The genome of A. trichopoda has the fewest WRKY TFs among the eight genomes studied. The genomes of the species representing recently evolved flowering plant clades have more variable WRKY TFs. Previously classified group and subgroup members in Arabidopsis were present in all species. The WRKYGQK has been modified into WRKYGKK or WRKYGEK in most species with fewer other variation.

Library of Congress Subject Headings

Angiosperms -- Genetics.
Transcription factors.
Genetic transcription.
Plant genetics.

Number of Pages



South Dakota State University



Rights Statement

In Copyright