Document Type

Thesis - University Access Only

Award Date


Degree Name

Master of Science (MS)

Department / School

Nutrition, Food Science, and Hospitality


The purpose of this study was to develop a rapid method for estimating carbohydrates available for fermentation and subsequent conversion to alcohol. Enzymatic and chemical conversions that parallel industrial ethanol production were explored. A rapid NIRS method was developed using AACC Method 76-13 as a reference procedure. This method yielded data on starch and free sugars measured as glucose. The sum of the free sugars and starch concentrations was referred to as total available carbohydrates. Two crop years, 1995 and 1996, involving a total of 1118 samples were assessed and predictive calibrations were developed using 181 samples. Calibrations equations developed for total available carbohydrates for ground corn yielded R2, SEC, and SECV, of 0.74, 0.98%, and 1.09%, respectively. Starch calibration equations for ground corn produced a R2 of 0.78. The SEC and SECV values were 0.87 and 0.92%, respectively. In general, ground corn calibrations yielded better predictive equations than nonground corn, as judged by R2, SEC, and SECV. Free glucose for which concentrations ranged from 0.19 to 2.12%, did not give acceptable NIRS calibrations.

Library of Congress Subject Headings

Corn -- Composition Carbohydrates -- Spectra Near infrared reflectance spectroscopy



Number of Pages



South Dakota State University