Thesis - University Access Only
Master of Science (MS)
Department / School
Biology and Microbiology
Gerald A. Myers
Purple coneflower, Echinacea purpurea (L.) Moench, portrays various medicinal properties including immunostimulatory effects. These medicinal qualities have been attributed to an isobutylamide named echinacein. Echinacea plants contain a network of oleoresin canals throughout the roots, floral buds, and seed achenes. This project involved a study of the development of oleoresin canals in the receptacles of buds of various stages of growth. Quantification of microscopic features was accomplished through the use of stereological methods. A semi-automatic image analysis system was utilized to obtain areal measurements of canal and receptacle areas. All data were analyzed with SAS, a computerized statistical analysis program. The canal area to receptacle area ratio was analyzed and a regression line indicated a direct relationship up to the maximum observed canal area. An inverse relationship was apparent in the oldest stage of growth. Analysis of floret development indicated a steady increase in canal volume during early stages of floret differentiation. At the time of pollen grain maturation, receptacle size increased significantly but canal volume leveled off. For maximum active substance per mass of tissue, harvest should occur when the most buds are in the microspore developmental stage, receptacle diameter 6.0mm - 7.0mm.
Library of Congress Subject Headings
Oleoresins -- Analysis
Echinacea (Plants) -- Therapeutic use
Herbs -- Therapeutic use
South Dakota State University
Muser, Jennifer K., "Image Analysis Study of Oleoresin Canals in Echinacea purpurea (L.) Moench" (1994). Electronic Theses and Dissertations. 54.